Resolution calculator (beta version)

Author: Dr. JJ Schmeidel
Version: 0.95 (last change 2017-03)
License: CC BY 3.0 or Beerware Rev. 42

TOS: Software/webpage/service is provided as is, without warranty of any kind. Author does not take any responsibility for anything connected with this software. Everything is subject to change, support can stop at any moment etc. By use of this software, user agrees to the Terms of service (Terms of use) of this software.

Script uses PHP, HTML, JavaScript languages. Following JavaScript addons are used: jQuery, jQuery-UI based on MIT License.

Camera data is property of Carl Zeiss Microscopy GmbH. Camera names are products and trademarks of Carl Zeiss Microscopy GmbH and other companies, respectively. This service has no official connection to the Carl Zeiss Microscopy GmbH.

Privacy: This page is not counting, tracking, watching, listening or anything tricky. It is also not calling any external servers. No sessions, statistics, nothing. Cookies only if you want to (if you want to save your config for later).

Configuration

Select objective magnification:	1x 1.25x 2.5x 5x 10x 16x 20x 25x 32x 40x 50x 60x other 63x 100x 150x 200x other 0.3x 0.4x 0.5x 0.63x 0.7x 0.75x 1x 1.5x 2x 2.3x 3.5x 4x
Select objective NA: (use dot as decimal separator)
Select camera:	MRc/MRm (old) Mrc5 (old) HRc/HRm (old) ICc1/ICm1 (old) ICc5 (old) ERc5 (old) 105 color 105 color R2 202 mono 208 color 305 mono/color 503 mono/color (old) 506 mono/color 512 mono/color (old) 702 mono 705 mono/color/pol 712 mono/color 807 mono/color 820 mono/color Orca Flash4 ImagEM X2 ImagEM X2-1k Evolve EMCCD CoolSNAP HQ2/ES2 pco.edge sCMOS ximea CMV2000 GENERIC 1
Select camera binning or scanning:	Scan 3x Scan 2x Standard Binn 1/2 Binn 1/3 Binn 1/4 Binn 1/8
Select camera adapter:	2.5x 1.6x video 1.2x SD 1x 0.8x NEW 0.65x Primostar 0.63x 0.5x 0.4x 0.33x video
Optovar / Stereo mag:	1x 1.25x 1.6x 2.5x 0.63x 0.65x 0.7x 0.75x 0.8x 1x 1.2x 1.25x 1.6x 2x 2.5x 3x 3.2x 4x 5x 6.3x 8x 10x 11.2x 12.5x 15x
Select light wavelength: [nm]
Resolution factor:	0.61 - real, Rayleigh 0.5 - theoretical 0.41 - confocal, 0.3 AU 0.37 - confocal, 0.25 AU
Sampling: [px/LP]	2 - optimal BIO, Nyquist 4 - optimal MAT, 2xNyquist 6 - a lot... 8 - overkill ???

Configuration saving

This saves the configuration in cookies on your computer. By clicking this button you agree to cookies.

Save Reset

Configuration sharing

This link contains above configuration. Just copy and send/publish this link as you like (yammer, facebook, etc) !

Config link:

Results

Total magnification (assuming 10x for oculars)
Selected camera info	Selected camera specifications.
Camera resolution	[px], or [mpix]
Camera pixel size	[um]
Camera chip size	[mm]
Required camera info	Theoretical camera specifications to fit objective characteristics.
Required camera resolution Required pixels in sensorplane (objective + chip size dep.)	[px], or required resolution [mpix]
Required camera pixel size (objective + chip size dep.)	[um]
Sampling ratio (based on horizontal chip pixel count) DEPENDS ON SAMPLING SETTING	[], information content of the camera image [um/px]
Interpretation DEPENDS ON SAMPLING SETTING
Camera field info (sample side)	Information about the object (camera image) obtained using system selected above.
Camera field pixel size (scaling pro camera pixel)	[um]
Camera field (size of one camera image)	[um], diameter [um], area [mm^2]
Resolution	Resolution calculation (magnification independent).
Depth of field Z-slice thickness (NA + wavelength dep.)	[um] (Z-optical thickness for n=1)
Optical lateral resolution (NA + wavelength dep.)	[um]
Resolution in sensorplane (NA + wavelength dep.)	[lp/mm]
Other	Additional calculations.
Measurable slope in confocal mic. (NA dep.)	[deg], not corrected for refractive index (so NA above 1 give error)
Theoretical image calculation for given camera (NOT WORKING, as visible on attached image)

Add camera

Temporarily adds generic camera.
Name: GENERIC 1, ResX: , ResY: , Pixel size: , Add generic camera

To Do's

objectives selection and saving (your microscope system)
Add Optovar, camera magnification changer
Condenser NA (transmitted light)
Stereo and Stereo mag/NA curves to calculate resolution
Refractive index for depth of field
Recheck the Z-resolution formula - DONE 0.72

Changes

0.95 total magnification corrected and rounded
0.94 added optovar/stereo switch
0.93 ratio rounded to two positions
0.92 added information content of the camera image
0.91 all units in um
0.90 sampling ratio interpretation corrected
0.89 sampling ratio normalized to 1
0.88 fixed wording
0.87 saving setup to cookies
0.86 sampling - works
0.85 cleaned up the code
0.84 resolution factor
0.84 removed one resolution box, only one needed
0.83 corrected resolution in sensorplane (was mag dependent)
0,82 NA input field accepts dot and comma
0.81 new cameras
0.8 descriptions of calculated fields
0.72 depth of field formula correction (/2)
0.71 interpretation color marking
0.69 interpretation
0.68 calculated fields - read only
0.67 new layout
0.61 link sharing works ! (tested in actual Chrome, Firefox and IE)
0.53 new cameras
0.52 fully works offline (tested)
0.51 added some php for databases
...
0.01 working proof of concept

Thanks

Thanks to all people who know better than me and helped me improve the calc. Thanks for discussions, new ideas, finding the bugs, clarify descriptions and correct calculations.
Great thanks to professionals from Goettingen, Jena and Muenchen.

References and sources

Carl Zeiss AcquisitionAdvisor v2.04 by Horst Schulze
http://zeiss-campus.magnet.fsu.edu/referencelibrary/dic.html
http://en.wikipedia.org/wiki/Optical_resolution
http://micro.magnet.fsu.edu/primer/anatomy/numaperture.html
http://www.mcb.arizona.edu/IPC/zoom_Page.htm
http://www.microscopyu.com/articles/optics/objectiveproperties.html
http://www.olympusconfocal.com/theory/resolutionintro.html

JJ © 2013-2016